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Lung viral load was determined at 72 h post-infection. Lungs of infected mice and control mice were homogenized in 1 mL of ice-cold PBS and frozen at −80°C for 14 h. Then, thawed samples were centrifuged at 2,000 × g for 10 min, and the supernatants were titrated by plaque forming assay as described above.
Copyright and License information: ©2019 Liu, Yan, Chen, Pan, Chen, Hong, Zhang, Liu, Wang and Chen.
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