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Zebrafish lines and husbandry

AH Alexandria N. Hughes
BA Bruce Appel
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All animal work was approved by the Institutional Animal Care and Use Committee at the University of Colorado School of Medicine. Zebrafish embryos were raised at 28.5 °C in embryo medium and staged according to hours or days post-fertilization (hpf/dpf) and morphological criteria. Tg(sox10:mRFP)vu234, Tg(sox10:tagRFP)co26, Tg(phox2bb:GAL4)co21, Tg(UAS:syp-eGFP)67, Tg(mbpa:tagRFP)co25, Tg(mbpa:eGFP-CAAX), and Tg(UAS:eGFP-CAAX)co18 transgenic lines were used. In addition, we generated and used the new lines Tg(cadm1b:GAL4)co53, Tg(dlg4b:GAL4)co54 (see section on enhancer trapping), and Tg(neuroD:sypb-mScarlet)co55. All other reporters were expressed by transient transgenesis to achieve sparse labeling for single cell analysis.

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