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Perfusion and tissue processing

YL Yanmin Luo
QX Qian Xiao
JW Jin Wang
LJ Lin Jiang
MH Menglan Hu
YJ Yanhong Jiang
JT Jing Tang
XL Xin Liang
YQ Yingqiang Qi
XD Xiaoyun Dou
YZ Yi Zhang
CH Chunxia Huang
LC Linmu Chen
YT Yong Tang
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During the following processes, all the experiments and data analyses were performed blind to treatment conditions. After the behavioral testing, under deep anesthesia with an i.p. injection of 1% sodium pentobarbital (4 mL/kg body weight), five rats from each experimental group were randomly selected and perfused transcardially with 4% paraformaldehyde. Brains were removed and split into two hemispheres by a midsagittal section. The right or left hemisphere from each rat was sampled at random and coronally sectioned into 60 μm sections on a cryostat microtome (CM1860, Leica). The sections were kept in anatomical series. From the sections containing the mPFC, every 6th section was sampled in a systematic-random manner with 15 sections per series on average. In the end, six sets of sampled sections were acquired.

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