DPPH Free Radical Scavenging Assay

EEAHR was tested for free radical scavenging activity by the DPPH method of Braca et al., [29]. Free radical scavenging activity of the extract was held based on the scavenging activity of stable DPPH. EEAHR in millipore water at different concentration (25-800μg) were added to each test tube and volume was made up to 2 ml using the millipore water. To this 3 ml of 0. 004% DPPH in 95% ethanol was added and the mixtures were incubated at room temperature under dark condition for 30 min. The scavenging activity on the DPPH radical was determined by measuring the absorbance at 517 nm.