Normally, miRNAs with similar expression patterns usually have functional correlation. To display the clustering information of defined set of miRNAs, heatmap analysis based on the miRNA expression value was conducted. While for the sample clustering, we took all miRNA TPM as input and used hcust in r to get the dendrogram.
Do you have any questions about this protocol?
Post your question to gather feedback from the community. We will also invite the authors of this article to respond.