Improve Research Reproducibility A Bio-protocol resource
- Home
- Protocols
-
Concise Method
Dual-luciferase reporter gene assay
Ask a
question
Favorite
Luciferase reporter gene assay was performed using the Dual-Luciferase Reporter Assay System (Promega Corporation, E1910) according to the manufacturer’s instruction. Luciferase reporter assays for ATG5, ATG7 and Linc02527, wild-type or mutant reporter constructs (termed WT or Mut; obtained from Genepharma) were cotransfected into HTR8 cells in 24-well plates with 100 nM MIR3185 or 100 nM miR-NC and Renilla plasmid by using Endofectin-Plus (Gene-Copoeia, Z01010A). All experiments were performed at least 3 times.
Copyright and License information: The Author(s) ©2018
Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.
Do you have any questions about this protocol?
Post your question to gather feedback from the community. We will also invite the authors of this article to respond.
Post a Question0 Q&A
