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In vitro growth in BHI broth and flagellar motility assays
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Sixteen-hour cultures of the indicated strains were diluted 1:20 in duplicate in BHI and grown with shaking at 37 °C. At the indicated times, the optical density at 600 nm (OD600) of the bacterial cultures was measured or an aliquot of bacterial cultures was collected to enumerate bacterial colony forming units (CFU). To analyze the flagellar motility of L. monocytogenes strains, 10403S, LS741 and LS743 were spotted on a soft agar plate (1% tryptone, 0.25% NaCl, and 0.3% agar) and incubated at 30 °C for 16 hours.
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