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Six independent cultures of M145 and 2L12 were grown in non-viscous medium (2xYT), and the morphology of mycelial pellets was analyzed using a Nikon Eclipse 6000 microscope. In order to measure the complete pellets of strain M145, consecutive bright-field images under 10× amplification were taken and stitched together using the ImageJ plugin Stitching (Preibisch et al., 2009) as pellets of the wild type were often too large to fit in a single field. The area of the cross-section of the pellet was measured using ImageJ tools.
Copyright and License information: ©2019 Arroyo-Pérez, González-Cerón, Soberón-Chávez, Georgellis and Servín-González.
This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
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