The genome sequences and predicted proteins of red abalone (Haliotis rufescens), Pacific abalone (H. discus hannai), and owl limpet (Lottia gigantea) were obtained from their respective data repository for comparison with the newly sequenced blacklip abalone. Each genome assembly was assessed for completeness with BUSCOv3 based on the metazoa odb9 lineage dataset (Waterhouse et al., 2017). Then, orthologous groups of proteins were identified between H. rufescens, H. discus hannai, H. rubra, and L. gigantea using an all-vs-all DIAMOND blastp search and Markov Cluster (MCL) clustering approach as implemented in OrthoFinder v2.2.7 (default setting). The computation and visualization of ortholog intersections across the four marine gastropods used UpSetR (Conway et al., 2017).
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