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2.9. Small interfering RNA transfection

KS Kazuhiro Shimazu
MI Masahiro Inoue
SS Shunsuke Sugiyama
KF Koji Fukuda
TY Taichi Yoshida
DT Daiki Taguchi
YU Yoshihiko Uehara
SK Sei Kuriyama
MT Masamitsu Tanaka
MM Masatomo Miura
HN Hiroshi Nanjyo
YI Yoshiharu Iwabuchi
HS Hiroyuki Shibata
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Small interfering RNAs for FN1 were obtained from OriGene Technologies, Inc. (Rockville, MD, USA) and included 3 types of FN‐1 siRNA: SR301640A (FN1‐A), SR301640B (FN1‐B), and SR301640C (FN1‐C); SR30004 (mock) was used as a negative control. The siRNAs were transfected with Lipofectamine RNAiMAX transfection reagent (Invitrogen, Tokyo, Japan) according to the manufacturer's protocol. The siRNAs were used at 100‐200 nmol/L concentration. Cells that were seeded or were in suspension were next lipofected for 24 hours.

Copyright and License information:  ©2018 The Authors. published by John Wiley & Sons Australia, Ltd on behalf of Japanese Cancer Association.
This is an open access article under the terms of the http://creativecommons.org/licenses/by-nc/4.0/ License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited and is not used for commercial purposes.

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