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Quantitative methylation analysis of DMRs in the candidate genes was performed on the 45 paired tumor and normal lung tissues by using the Sequenom MassARRAY platform (CapitalBio) as described previously [20]. This platform employs RNA base-specific cleavage and matrix-assisted laser desorption/ionization time-of-flight mass spectrometry. PCR primers for MassARRAY were designed by using Epidesigner (http://www.epidesigner.com) (S3 Table). The spectra methylation ratios were obtained via Epityper software version 1.0 (Sequenom, San Diego, CA, USA).
Copyright and License information: ©2018 Wang et al
This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
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