Time required to induce dormancy

NG Nafiseh Geshnizjani
FG Farshid Ghaderi-Far
LW Leo A J Willems
HH Henk W M Hilhorst
WL Wilco Ligterink
request Request a Protocol
ask Ask a question
Favorite

Seeds of MM were subjected to 37 °C for 6, 12, 18, 24, 30, 36, 42, 72, 96, 120 and 144 h. Subsequently, they were transferred to 25 °C for 14 days. Afterwards, the normal germinated seeds were counted manually for each time point. A segmented model was fitted to seed germination (percentage) versus time (hours) using the NLIN procedure of the SAS software [51]:

where y is germination percentage, x is days at 37 °C, b is model slope line, x0 is the start time of dormancy induction.

Do you have any questions about this protocol?

Post your question to gather feedback from the community. We will also invite the authors of this article to respond.

post Post a Question
0 Q&A