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BV-2, EOC-20, and WI-38 cells were seeded at 5,000 cells/well and RPMI 8226 cells were seeded at 10,000 cells/well in 96-well plates. Following overnight incubation, cells were treated with carfilzomib, ONX0914 or YU102 at indicated concentrations for 72 h. Cell viability was determined by CellTiter 96 AQueous One Solution Cell Proliferation assay (Promega) following the manufacturer’s protocol. Absorbance at 490 nm was measured using a SpectraMax M5 microplate reader (Molecular Devices).
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