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For Western blot and immunostaining of tissue sections, we used specific antibodies which had been well characterized before, such as AQP2 (H7661) (Nielsen et al., 2006), pS256-AQP2 (K0407) (Christensen et al., 2000), CaMKII (ab34703, Abcam, Cambridge, United Kingdom), β-actin (Cell Signaling Technology, Danvers, MA), ERα (sc-787, Santa Cruz, Texas, USA), and ERβ (sc-390243, Santa Cruz, Texas, USA). Polyclonal goat anti-rabbit IgG horseradish conjugated secondary antibodies were used [P448] (Dako, Glostrup, Denmark).
Copyright and License information: ©2019 Tingskov, Choi, Holst, Hu, Li, Wang, Frøkiær, Nejsum, Kwon and Nørregaard.
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