Antibodies

The following antibodies were used: sheep anti-uromodulin (K90071C, Meridien Life Science Inc., Cincinnati, OH; 1:500 for WB and 1:300 for IF), sheep-anti uromodulin (ab9029, Abcam; 1:500 for WB), rabbit anti-hepsin (100022, Cayman Chemical, Ann Arbor, MI; 1:50 for IF), mouse anti-flotillin-1 (BD 610821, BD Biosciences, Franklin Lakes, NJ, 1:500 for WB), rabbit anti-GAPDH (2118, Cell Signaling Technology, Danvers, MA; 1:500 for WB), mouse anti-βactin (A5441, Sigma-Aldrich; 1:10 000 for WB), rabbit anti-calnexin (C4731, Sigma-Aldrich; 1:300 for IF), rabbit anti-phospho NKCC2 (R5, phosphorylated Thr96 and Thr101)(gift from Prof. Biff Forbusch, Yale; 1:500 for WB), rabbit anti-phospho NKCC2 (phosphorylated Thr96) (gift from Prof. Jan Loffing, Zurich; 1:300 for IF), rabbit anti-NKCC2 (AB3562P, Merck-Millipore, 1:500 for WB and 1:100 for IF), rabbit anti-phospho NCC (phosphorylated Thr53)(gift from Prof. Jan Loffing; 1:5000 for WB), rabbit anti-NCC (AB3553, Merck-Millipore, 1:500 for WB), rabbit anti-aquaporin 1 (AB2219, Merck Millipore; 1:500 for WB), rabbit anti-aquaporin 2 (A7310, Sigma-Aldrich; 1:1000 for WB), anti-phospho SPAK (Ser373)/phospho OSR1 (Ser325)(07–2273, Merck Millipore; 1:500 for WB and 1:300 for IF), rabbit anti-SPAK (07–2271, Merck Millipore; 1:500 for WB), mouse anti-GM130 (ab169276, Abcam, Cambridge, UK; 1:300 for IF), rabbit anti-NGAL (ab63929, Abcam; 1:500 for WB), rabbit anti-GRP78 (ab21685, Abcam; 1:500 for WB and 1:300 for IF).