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Validation of point mutations by PCR and sanger sequencing
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The reliability of the exome analysis and somatic variant identification strategies was assessed using PCR and Sanger sequencing. PCR was performed using the GeneAmp PCR System 9700 (Applied Biosystems, Foster City, CA, USA). About 20 ng template DNA from each sample was used per reaction. The products were sequenced, and all sequences were analyzed with the Sequencing Analysis Software Version 5.2 (Applied Biosystems).
Copyright and License information: The Author(s). ©2018
Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.
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