Improve Research Reproducibility A Bio-protocol resource
Concise Method
tooltip

In vivo tumor xenograft model

HM Hakm Y. Murad
EB Emma P. Bortz
HY Heng Yu
DL Daishen Luo
GH Gray M. Halliburton
AS Andrew B. Sholl
DK Damir B. Khismatullin
request Request a Protocol
ask Ask a question
Favorite

The four-week-old male and female Nu/Nu athymic nude mice were purchased from Jackson labs (J:NU 007850, Bar Harbor, ME) and allowed one week of acclimation. A 200 μL bolus containing 1.0 × 106 Hep3B cells in PBS and Matrigel Matrix High Concentration (Corning, Corning, NY) mixed in a 1:1 volume was subcutaneously injected on the left and right flanks of each animal via a 28-gauge needle. Digital calipers and a SonoSite 180+ ultrasound imaging system (SonoSite, Bothell, WA) were used to measure tumor dimensions. The tumor volume was calculated using the following formula: V = π/6 × 2a × b, where a is the short axis and b the long axis of the tumor. Once the tumor volume reached approximately 200 mm3, mice were randomly assigned to a specific treatment group. There was a total of 10 tumors per group.

Copyright and License information: The Author(s) ©2019
Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

Do you have any questions about this protocol?

Post your question to gather feedback from the community. We will also invite the authors of this article to respond.

post Post a Question
0 Q&A