Improve Research Reproducibility A Bio-protocol resource
- Home
- Protocols
-
Ask a
question
Favorite
Cell pyroptosis was measured with FAM-FLICA Caspase-1 Assay Kits (ICT, USA). According to the manufacturer's instructions, cells were incubated with 40 μl phosphate buffered saline (PH = 7.4) containing 10 μl FLICA at 37°C protected from light for 30 min and 5 μL propidium iodide. Cell pyroptosis was analyzed on a FACScalibur flow cytometer (BD Biosciences, San Jose, USA). The double-positive cells were considered to be pyroptotic cells.
Copyright and License information: ©2019 Wang, Zhu, Yuan, Wen, Liu, Wang, Qu, Li, Liu, Sun and Liu.
This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
Do you have any questions about this protocol?
Post your question to gather feedback from the community. We will also invite the authors of this article to respond.
Post a Question0 Q&A
