Bone histomorphometric static parameters were assessed as presented in a recent report from our group.34 In brief, a 400-µm × 600-µm ROI located 150 µm below the growth plate of the femoral metaphysis (n=6 for each) was used for assessing the following static parameters: bone volume/tissue volume (BV/TV), trabecular number (Tb.N), trabecular thickness (Tb.Th), and trabecular separation (Tb.Sp). Whenever possible, abbreviations and calculations followed the guidelines of the American Society for Bone and Mineral Research (ASBMR) Histomorphometry Nomenclature Committee.35
Do you have any questions about this protocol?
Post your question to gather feedback from the community. We will also invite the authors of this article to respond.
Tips for asking effective questions
+ Description
Write a detailed description. Include all information that will help others answer your question including experimental processes, conditions, and relevant images.