Isolation of primary hepatocytes and hepatic stellate cells

SK Sabine Klein
CK Carola-Ellen Kleine
AP Andrea Pieper
MG Michaela Granzow
SG Sebastian Gautsch
MH Mimoun Himmit
KK Katharina Kahrmann
RS Robert Schierwagen
FU Frank Erhard Uschner
FM Fernando Magdaleno
MN Maria Eleni Naoum
GK Glen Kristiansen
TW Thomas Walther
MB Michael Bader
TS Tilman Sauerbruch
JT Jonel Trebicka
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Primary rat hepatic stellate cells were isolated and cultured as previously described8,35. Viability and purity were routinely >95%. For early activation of HSCs, cells were harvested on day 10.

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