In vivo bioluminescence imaging

Tumor growth and response to treatment were assessed longitudinally by in vivo BLI with 150 mg/kg D-Luciferin injected intraperitoneally (i.p). Imaging was initiated within five minutes and sequential images were acquired at 3-minute intervals until peak BLI signal was reached for each animal. In the mice with BCBM, treatment effect was assessed by calculating changes in tumor bioluminescence at each time-point relative to the BLI intensity recorded for that mouse at baseline (PID10).