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DPP4 activity in plasma and in tissue lysates was assayed as described earlier (3). Briefly, DPP4 activity was determined as the rate of 7-amino-4-methylcoumarin (AMC) cleavage per minute per mL from the synthetic substrate H-glycyl-prolyl-AMC (Sigma Aldrich, St. Louis, MO, USA). AMC fluorescence (excitation/emission-380/460 nm) was measured in a plate reader (Synergy H1 multi-mode microplate reader; Biotek, Winooski, VT, USA).
Copyright and License information: ©2019 Sarkar, Nargis, Tantia, Ghosh and Chakrabarti.
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