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The procedures of SDS-PAGE and immunoblotting were described as our previous studies (19). Briefly, to measure the expression of target molecules, proteins were acquired from cell lysates and quantified by BCA assay. Anti-mouse-IL-6 and anti-NFκB were from Abcam. Anti-A-FABP was from R&D system. Other antibodies, including anti-STAT3, anti-phospho-STAT3 (Tyr705), β-actin, GAPDH, were from Cell Signaling Technology.
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