Mice.

Tie2-Cre;Etv2-CKO, VEC-Cre;Etv2-CKO, and Vav-Cre;Etv2-CKO mice were generated as described previously (32, 33). C57BL/6J mice were used as wild-type mice. Tie2-Cre;Etv2^f/f mice were used as Tie2-Cre;Etv2-CKO mice. VECadherin-Cre;Etv2^f/f mice were used as VECadherin-Cre;Etv2-CKO mice. Vav-Cre;Etv2^f/f mice were used as Vav-Cre;Etv2-CKO mice. Littermate subjects were used as controls with the CKO mice. Both male and female mice were used in an equal quantity in any given experiment. The ages of the experimental animals were between 10 and 12 weeks. Animals were randomly distributed into groups when different treatments, such as Etv2 shRNA or Gfp shRNA treatment, N-acetyl cysteine plus apocynin (NAC+APO) or vehicle treatment, and Etv2 siRNA or scrambled siRNA nanoparticle treatment, were employed. A group of technicians was in charge of distributing and codifying the experimental animals. The investigators were not aware of the group allocation until the treatment, data collection, and data analysis were done.