Improve Research Reproducibility A Bio-protocol resource
- Home
- Protocols
-
Request a Protocol
Ask a
question
Favorite
At 2 days postransduction, cell culture medium was removed, and cells were lysed in 20 μl per well of 1× reporter lysis buffer (Promega) and then frozen at −80°C. After thawing, firefly luciferase (ffLuc) expression was measured in relative light units per second on a Synergy H1 Hybrid Multi-Mode microplate reader using 100 μl luciferin buffer (200 mM Tris [pH 8], 10 mM MgCl2, 300 μM ATP, 1× firefly luciferase signal enhancer [catalog number 16180; Thermo], and 150 μg/ml d-luciferin).
Copyright and License information: ©2018 American Society for Microbiology.
All Rights Reserved.
Do you have any questions about this protocol?
Post your question to gather feedback from the community. We will also invite the authors of this article to respond.
Post a Question0 Q&A
