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Up to 1×106 MDA-MB-231 cells per well were plated in 6-well plates. Cells were incubated overnight, and 3 parallel wounds per well with the same interval were created using a pipette tip. Images were captured at 0, 12 and 24 h after wounding. The distance migrated by the cells to close the wounded area during the time was measured using ImageJ software. The results are expressed as the wound-healed rate (%), that is, the distance migrated by cells at certain time divided by the wound distance at 0 h. Experiments were performed in duplicate and repeated at 3 times.
Copyright and License information: ©2016 Zhang et al
This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
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