To study the effect of rhHPPCn in acute liver injury, male Balb/c mice (20–22 g) were randomly grouped (eight in each group, n = 8). A single intraperitoneal injection of 20% CCl4 (in olive oil) in a volume of 5 ml/kg body weight was used to establish acute liver injury in the mice model. An equal volume of olive oil was intraperitoneally injected in Balb/c mice to establish the control group.
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