Cells and Virus

C. auratus gibelio caudal fin (GiCF) cell line was established in our previous work (Lu et al., 2018a), cells were grown in M199 medium (Gibco, USA) with 10% fetal bovine serum (Gibco, USA) and antibiotics (100 U penicillin ml⁻¹ and 100 mg streptomycin ml⁻¹) at 25°C. Hela cells were cultured in MEM Medium (Gibco, USA) supplemented with 10% fetal bovine serum (Gibco, USA) at 37°C and 5% CO₂. The CyHV-2 strain was isolated from infected C. auratus gibelio samples cultured in Sheyang City, Jiangsu Province, China (Xu et al., 2014).