SKOV-3 cells (1×106 cells/well) were seeded into 6-well plates and cultured to a 100% confluent monolayer. In each well a wound was induced by scratching a straight line into the cell layer with a 200 µl pipette tip. After 24 h of incubation with RPMI 1640 medium without FBS, images of the wound healing areas were captured and measured with a Leica DM2500 image analysis system (Leica, Mannheim, Germany).
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