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RNA was coextracted together with DNA from fresh peat soil (four extractions) and from microcosms (one extraction for each replicate) by bead-beating lysis, organic solvent extraction and precipitation (Griffiths et al., 2000). DNA was removed from RNA/DNA coextracts using RNase-free DNase (Promega, Mannheim, Germany) according to the manufacturer's instructions.
Copyright and License information: ©2016 International Society for Microbial Ecology
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