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MCF10DCIS cells were grown in three dimensional Matrigel cultures as described [38]. Briefly, 3x103 cells were suspended in modified growth medium containing 2% growth factor-reduced Matrigel, 2% Horse serum (Cellgro) and 5ng/ml EGF (R&D systems). Cell mixture was plated on top of a solidified layer of growth factor-reduced Matrigel. Cells were fed every 4 days. Phase contrast images were acquired using the Nikon Eclipse Ti microscope.
Copyright and License information: ©2016 Henry et al.
This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
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