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Reconstruction of the Key lncRNA-miRNA-mRNA Subnetwork

LT Lichan Tao
LY Ling Yang
XH Xiaoli Huang
FH Fei Hua
XY Xiaoyu Yang
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Every lncRNA and its related miRNAs and mRNAs in the global triple network were extracted to construct a new subnetwork using Cytoscape software. The number of related lncRNA-miRNA-mRNA triplets was calculated. By comparing the node degree of lncRNA and the number of related lncRNA-miRNA and miRNA-mRNA pairs, the target lncRNAs were identified. We then performed qRT-PCR to confirm the changes of lncRNA in samples from DCM patients and healthy controls. For further analysis, GO and KEGG enrichment analyses were performed for each of the validated lncRNAs by using their mRNA neighbors in the lncRNA-miRNA-mRNA subnetwork.

Copyright and License information:  ©2019 Tao, Yang, Huang, Hua and Yang
This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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