Subcellular localization assays

The full-length cDNA sequences of GmMYB118 were fused to the N-terminus of the hGFP gene (MYB118-GFP, Additional file 1: Table S2) under the control of the CaMV 35S promoter. The cDNA coding sequences of AtWRKY25 (At2g30250) that Located in the nucleus [29] were fused to the N-terminus of the mCherry gene (WRKY25-RFP, Additional file 1: Table S2) under the control of the CaMV 35S promoter. The recombinant plasmid of GmMYB118-GFP and AtWRKY25-RFP were cotransformed into Arabidopsis protoplasts via the PEG4000-mediated method [18, 55]. The expression of the fusion protein was observed under dark conditions for 12 h, and GFP and RFP was detected by laser scanning confocal microscopy (Zeiss LSM 700, Germany) [18, 30].