Improve Research Reproducibility A Bio-protocol resource
- Home
- Protocols
-
Ask a
question
Favorite
The astrocytes (2.5 × 105 cells/well) were cultured in normal glucose (5.5 mM) and high glucose (25 mM) DMEM (10% FBS) to a monoconfluent layer in 6-well cell culture plates. Using sterile 200 µl pipette tip scratches were made on the cell layer, the plates were then rinsed with sterile PBS to remove cell debris and replaced with fresh cell culture media. At 0, 24 and 48 hrs after scratch, cells were stained with Calcein AM (10 µM), fluorescent images were obtained randomly using a Zeiss fluorescence microscope.
Copyright and License information: ©2018 Li et al.
This is an Open Access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution and reproduction in any medium provided that the original work is properly attributed.
Do you have any questions about this protocol?
Post your question to gather feedback from the community. We will also invite the authors of this article to respond.
Post a Question0 Q&A
