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The method of ROS burst measurement assay was as described [16]. Four leaf discs (4 mm in diameter) per individual genotype were collected into 96-well plates containing sterile water and recovered overnight. The next day, the sterile water was replaced by a solution containing 20 μm/L luminol L-012 (Sigma-Aldrich), 1 μg/mL horseradish peroxidase (HRP, Sigma-Aldrich), and 100 nm/L flg22, 1 μM RALF1 (or without RALF1 in control). Luminescence was measured for the indicated time period using Fluoroskan Ascent FL (Thermo Scientific).
Copyright and License information: ©2018 Li et al
This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
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