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Precision-cut liver slices

MO Marta Olivares
AN Audrey M. Neyrinck
SP Sarah A. Pötgens
MB Martin Beaumont
NS Nuria Salazar
PC Patrice D. Cani
LB Laure B. Bindels
ND Nathalie M. Delzenne
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After 1 week of acclimatisation, mice in experiment 2 were anesthetised with ketamine (100 mg/kg body weight) (Nimatek; Eurovet, Bladel, Netherlands) and xylazine (100 mg/kg body weight) (Rompun; Bayer, Leverkusen, Germany). Precision-cut liver slices (PCLS) were prepared as previously described [32, 33]. PCLS were incubated without (control medium) or with vildagliptin (0.6 mg/ml) in oxygenated and supplemented Waymouth’s medium for 4 h at 37°C under agitation. PCLS were frozen in dry ice until analysed.

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