MYC IHC Stain and Variables Predictors Stability

Immunohistochemistry technique relies on numerous preanalytical and analytical variables that influence the immunoreactivity of proteins. As some antibodies require freshly diluted antibody or freshly cut unstained slides, the stability of MYC antibody dilution over 6 weeks was verified as well as antigen preservation within unstained thin section over time. The same onslide controls [1 BL (MYC+) and 1 DLCBL (MYC−)] were simultaneously submitted to variable preanalytical conditions including incremental delay after block sectioning, various aging antibody dilution, or combination of both. The goal was to see whether the MYC IHC stain would remain stable within a timeframe of 6 weeks, and if not, to measure the influence, if any, of stain variability on ratio EMST, CV, and percentage of positive MYC IHC nuclei.