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Cells were harvested, washed with phosphate-buffered saline and fixed with 95% ice-cold methanol for 30 min at 4°C.14 The cells were then seeded on the slide and allowed to dry in the air. Next, the cells were stained with Wright-Giemsa for 5 min and rinsed in deionized water. Finally, coverslips were fixed with Permount prior to microscopy (Nikon, Tokyo, Japan).
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