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Colony formation assay and in vitro apoptosis analysis

YX Yi Xu
XY Xiaoyu Yang
DG Di Gao
LY Liu Yang
KM Keith Miskimins
SQ Steven Y. Qian
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Cancer cell survival and apoptosis after various treatments were assessed by a colony formation assay and an Annexin V Apoptosis Detection Kit I, respectively, as described elsewhere [25–28]. In the colony formation assay in vitro, survival fraction = plating efficiency in treatment group/plating efficiency in control group. An Accuri C6 flow cytometer was used for apoptosis analysis (10,000 cells were counted for each sample). Unstained cells and cells stained with FITC Annexin V only or PI only were used to set up compensation and quadrants. Data were analyzed by FlowJo software.

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Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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