Animals and reagents

This study was approved by the Institute Research Medical Ethics Committee of the First Affiliated Hospital of China Medical University. All rats in the study were used strictly in accordance with the National Institutions of Health Guide for the Care and Use of Laboratory Animals. Seventy-five eight-week-old female SD rats weighing 180-220 g were obtained from the Experiment Animal Center, China Medical University. DSS was purchased from MP Biomedicals; TGF-β1, rat IL-17A, IL-10 and Flow cytometry staining Kit for Tregs were purchased from Platinum Company; Trizol reagent was purchased from Invitrogen Company; ELISA kit was bought from eBioscience; reverse transcription and real-time PCR kits were purchased from BioTeke Corporation, Beijing, China; Western blot kit was purchased by Beyotime; the primers for Foxp3 were synthesized by Invitrogen Company and the primary antibody was purchased from CST Company. Other necessary reagents were available in our laboratory.