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Cells were immunohistochemically identified with the following markers: CD20 for B lymphocytes, CD138 for plasma cells, CD4 or CD8 for T lymphocytes, CD56 for NK cells, and FoxP3 for T regulatory cells. Macrophages were immunofluorescence-stained with an anti-human CD68 antibody and pSTAT1 or cMAF, which are transcription factors localized in the nuclei of M1 and M2 macrophages, respectively (6). All antibodies used and their respective dilutions are summarized in the Supplementary Table 1.
Copyright and License information: ©2019 Aguado-Domínguez, Cabrera-Pérez, Suarez-Benjumea, Abad-Molina, Núñez-Roldán and Aguilera.
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