Western blot analysis

Western blot analysis was conducted as previously described [13]. Primary antibodies against phospho-FLT3 (Tyr591) (#3461) and phospho-AURKA (Thr288) (#3079) were purchased from Cell Signaling Technology. The anti-FLT3 antibody (sc-480) and anti-AURKA antibody (07–648) were purchased from Santa Cruz Biotechnology and Upstate, respectively. The anti-β-actin rabbit polyclonal antibody (GTX110564) was purchased from GeneTex (CA, USA). The secondary antibodies horseradish peroxidase (HRP)-linked goat anti-rabbit IgG (111-035-003) were purchased from Jackson Immuno (West Grove, PA, USA). MV4-11 cells were incubated with compound BPR1K871 for 2.0 h at the indicated concentrations. Cell lysates were prepared and analyzed by immunoblotting. For AURKA analysis, the cell lysates were obtained from MV4-11 cells incubated for 16 h with 40 ngmL^-1 nocodazole, followed by drug treatment for 2.0 h at the indicated concentrations.