TUNEL Staining of Tumor Tissue Sections

Tumor tissue sections (5 μm thickness) were prepared and stained with hematoxylin and eosin (H&E) according to standard protocols and then assessed for apoptosis. In situ cell death was determined by TUNEL staining of tumor tissue sections (paclitaxel-treated and nontreated human breast carcinoma tissues). The TUNEL assay was performed in accordance to the manufacturer’s instructions (in situ cell death detection kit; catalog # G3250, Roche Applied Science, Germany) to demonstrate DNA fragmentation.