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CFU assays were performed by plating 5 × 103 cells/well from single cells obtained from day 12 of co-culture into methylcellulose H4435 in a 24-well plate. Cells were incubated at 37 °C for 14 days before the colonies were counted based on standard morphological criteria. BFU-E (burst-forming unit-erythroid), CFU-E (colony-forming unit-erythrocyte), CFU-GM (colony-forming unit-granulocyte/macrophage), and CFU-GEMM (colony-forming unit-granulocyte/erythroid/macrophage/monocyte) were classified and enumerated based on morphological recognition.
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Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.
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