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Histopathological and immunohistochemical (IHC) analyses
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Back skin tissues of mice (1 cm2) were collected, fixed with neutral buffered formalin (NBF) for 24 h, and embedded in paraffin. Paraffin blocks were cut into 3 μM thick sections and stained with hematoxylin and eosin (H&E) using a standard protocol (Abion CRO, Seoul, Korea). Epidermal thickness was measured at the thickest part of five different dorsal epidermis specimens per mouse group, and significant differences between each pair of conditions were analyzed.
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Open Access This article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.
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