Improve Research Reproducibility A Bio-protocol resource
- Home
- Protocols
-
Request a Protocol
Ask a
question
Favorite
LDH release by SH-SY5Y cells was examined using a Cytotoxicity Detection Kit (Roche, Manheim, Germany) according to the manufacturer's instructions. Briefly, 100 μl of culture supernatant was collected from each group and transferred to a 96-well plate. After adding 100 μl of reaction solution to each well, the samples were incubated for 30 min at room temperature in the dark. The absorbance at 490 nm was then measured to determine the amount of released LDH.
Copyright and License information: ©2019 Jing, Yang, Fu, Wang, Yang, Yuan, Chen, Ding, Chen and Tian.
This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
Do you have any questions about this protocol?
Post your question to gather feedback from the community. We will also invite the authors of this article to respond.
Post a Question0 Q&A
