Improve Research Reproducibility A Bio-protocol resource
- Home
- Protocols
-
Concise Method
Intradermal Injection of Interleukin 23 (IL-23)
Request a Protocol
Ask a
question
Favorite
Mice were anesthetized and PBS (20 μL), either alone or containing recombinant mouse IL-23 (500 ng; Biolegend, San Diego, CA, USA), was injected intradermally into the ears, using a 30 gauge needle, for five consecutive days. Ear thickness was measured prior to injection at day 0, and thereafter, on days following cytokine injections, using a dial thickness gauge (Jiuliang, Shanghai, China). All tests were performed blinded.
Copyright and License information: ©2019 Zheng, Liang, Xu, Li, Luo, Wu, Li, Tang, Zhong, Chen, Yang, Sun, Zhang and Xu.
This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
Do you have any questions about this protocol?
Post your question to gather feedback from the community. We will also invite the authors of this article to respond.
Post a Question0 Q&A
