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Forskolin Treatment of miR-142-3p Overexpressed MTregs

GD Gerjan J. Dekkema
TB Theo Bijma
PJ Pytrick G. Jellema
AB Anke Van Den Berg
BK Bart-Jan Kroesen
CS Coen A. Stegeman
PH Peter Heeringa
WA Wayel H. Abdulahad
JS Jan-Stephan Sanders
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In order to assess if cAMP elevating agent could restore Treg function, miR-142-3p overexpressed MTregs were cultured in the presence and absence of 0.1 μM Forskolin (Sigma Aldrich, Saint Louis, Missouri, USA). Next, intracellular cAMP levels and suppressive capacity were assessed at 48 and 72 h, respectively.

Copyright and License information:  ©2019 Dekkema, Bijma, Jellema, Van Den Berg, Kroesen, Stegeman, Heeringa, Abdulahad and Sanders.
This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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