Goat Pre-Implantation Stages Material

Female Anhui white goats (AWGs) were farm-raised by the Boda Company (Baogong Town, Feidong County, Hefei, China) under a unified field management system. All experimental animals were estrus-synchronized by treatment with EAZI-Breed CIDR (CIDR, Hamilton, New Zealand) for 12 days and superovulated prior to CIDR removal. The estrus test was performed 12h after stopping CIDR, and artificial insemination was performed on the female AWGs that were estrus at the same time. After 36–48, 56–60, 87–92, 97–100, and 109–112 h of mating, oocytes and 2-cell, 4-cell, 8-cell, and 16-cell cells were flushed from the oviduct. Morulae and blastocysts were obtained from the uterus after 152–156 and 212–218 h, respectively. A total of 21 samples were obtained in these seven stages, and each stage of the sample had three replicates. Oocytes and pre-implantation cells were washed several times in 1% DBPS solution. Five obtained oocytes and pre-implantation cells at each stage were pooled and snap frozen in liquid nitrogen.