Disk Diffusion Method

The antibiotic susceptibility profile of the clinical isolate of A. baumannii was re-checked against the values previously reported by Machado et al. (2018). This was conducted using the Kirby Bauer disk diffusion method as per CLSI guidelines (Cinical Laboratory Standards Institute [CLSI], 2013). Briefly, agar plates were lawned with bacteria diluted to OD₆₀₀ ∼ 0.1. Antibiotic disks were placed onto the agar surface and allowed to incubate overnight at 37°C. The zone of inhibition (ZOI) produced by the antibiotic was then used as a measure of susceptibility or resistance of the bacteria to the antibiotic according to the breakpoints outlined in the EUCAST guidelines (The European Committee on Antimicrobial Susceptibility Testing [EUCAST], 2019).